OMB Meeting Book_9-11-14

10/1 - Overview of Performance Data (SLV & MLT) vs SMPR for SPIFAN samples

Parameter

SLV (Sept. 2012)

MLT (July. 2014)

AOAC SMPR 2012.011

All forms of infant, adult, and/or pediatric formula (powders, ready-to-feed liquids, and liquid concentrates

Matrices

12 SLV Test Matrices Kit

6 SLV Test Matrices Kit

Quantitation limit (g/100 g)

0.001*

0.001*

≤ 0.001*

Quantitation range (g/100 g)

0.001* - 7.94*

0.001* - 7.94*

≤ 0.001* - 8.00*

Recovery (%)

100.0 – 102.9 % (C18:2/C18:3)

90 – 110 %

RSD (repeatability) (%) r

≥ 3.0* = < 1.3 < 3.0* & ≥ 0.5* = < 1.7 < 0.5* & 0.05*= < 1.8

≥ 3.0* = 2.5 (1.1-4.4) < 3.0* & ≥ 0.5* = 2.8 (1.2-4.8) < 0.5* = 2.1 (1.4-13.0**)

≥ 3.0* = ≤ 2 < 3.0* & ≥ 0.5* = ≤ 4 < 0.5* = ≤ 7.0

< 0.05* & ≥ 0.005* = < 3.5 < 0.005* & ≥ 0.001* = < 4.1

RSD (intra lab precision) (%) iR

≥ 3.0* = < 1.1 < 3.0* & ≥ 0.5* = < 1.7 < 0.5* & 0.05*= < 2.9

Not done

Not specified

< 0.05* & ≥ 0.005* = < 6.3 < 0.005* & ≥ 0.001* = < 7.0

RSD (reproducibility) (%) R

Not done

≥ 3.0* = 5 (4.9-5.3) < 3.0* & ≥ 0.5* = 5.0 (1.9-9.6) < 0.5* = 5.8 (2.7-23.5**)

≥ 3.0* = ≤ 4 < 3.0* & ≥ 0.5* = ≤ 8 < 0.5* = ≤ 15

All results expressed in g/100 g reconstituted final product / Concentrations apply to: a) ‘ready-to-feed” liquids “as is”; b) re-constituted powders (25 g into 200 g of water); and c) liquid concentrates diluted 1:1 by weight. NIST reconstitution rate 1 : 10

** trans fatty acid results