OMB Meeting Book_9-11-14
COMMENT PARAMETERS
USER COMMENTS
AOAC OMA
2012.22
Method Name/Manuscript Title
Vitamin C in Infant Formula and Adult Nutritionals by UPLC‐UV
Manuscript Reference In your experience using the method, does the method perform according to the method’s applicability as written? In your experience with the method, is there any safety concerns identified while using or regarding use of the method? Do you have any information that supports regarding the reproducibility of the method as written: If so, please specify and submit information? Please attach any documentation to support your feedback, if available. Based on your experience with the method, are there any recommended changes to the AOAC First Action Method as written?
As Vitamin C is unstable and oxidized to dehydroascorbic acid, method suggests use TCEP as a reducing agent. Dehydroascorbic acid which has very poor response on UV may show decrease in the peak area of ascorbic acid after keeping for long time. LC column with 1.7 µm particle size may create problem of backpressure after few injections due to sample matrix.
Even though after using the TCEP as a reducing agent need to check stability of the standards and sample extract. And should be mentioned in the method. (Watchful for the response of standards and sample extract.)
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