OMB Meeting Book_9-11-14
12 ( 2 ) At each working standard concentration, the peak areas of standards injected at the beginning and end of a set of samples should not increase or decrease by more than 10%. ( e ) Calculation of vitamin B concentrations in samples.— The vitamin B concentration in each injected sample preparation is
12
extrapolated from the vitamin B The concentration of vitamin B
standard curve prepared above. in each product can then be 12
12
calculated.
C = C × D ss × D V p i 1 2
p where C = product concentration in g/kg; C = vitamin B concentration of the injected sample preparation extrapolated from standard curve in g/L; D = volume of the first dilution in mL (100 mL); ss = sample size in g; D = volume of the second (final) dilution in mL; V = volume of filtrate loaded onto the cartridge in mL. For each set of samples, the control result must be within 3 standard deviations of the control mean. i 12 1 2
G. Validation Data Note: SLV and MLT data from the ERP checklist will be added to this section. Data tables will be added by September 19.
References: J. AOAC Int . 95 , 313(2012); AOAC SMPR 2011.005 J. AOAC Int . 95 , 293(2012)
© 2012 AOAC INTERNATIONAL
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