AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 27. NIST SRM 1849a, Total Carnitine n = 12

Calculated Amount

Mean

SD

(mg/100g)

(mg/100g)

(mg/100g)

%RSD r

14.8 15.6 15.5 16.0 15.8 14.5 15.0 14.9 15.1 15.7

15.3

0.44

2.9

15.4

0.81

5.3

15.0

0.10

0.7

15.4

0.26

1.7

15.2 15.3 Mean (mg/100g)

Certified Mass Fraction Mean / Range (mg/100g)

%RSD r

15.3

NA

3.2

Recommendations It is the recommendation of the Study Director the method is fit-for-purpose in determining total choline and L-carnitine in infant formula and adult/pediatric nutritional formula and that it be adopted as AOAC First Action Official Method status. Acknowledgements The Study Director would like to thank Jeff Shippar who developed the LC/MS/MS conditions for this method. The Study Director would also like to thank Justin Gilmore who was a major part in completing the SLV and contributing modifications to make a more efficient and rugged method overall. Thank you to Katherine Nass, Tom Shook, and Brian Smith who contributed to the collection of data over the course of the SLV.