AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2015.10 (Jan. 2019) Carn-07 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix II. Standard Method performance requirements (SMPRs) Method Performance Requirements for Total Choline (SMPR 2012.013)

Analytical range

2-250 a

Limit of detection (LOD) Limit of quantitation (LOQ) Repeatability (RSD r )

0.7 a 2.0 a

2 a

≤10%

20 a

100 a 200 a

≤5%

Recovery

90 to 110% of mean spiked recovery over the range of the assay

Reproducibility (RSD R )

2 a

≤15%

20 a

≤10% 100 a 200 a a mg/100 g Concentrations apply to: (1) “ready-to-feed” liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. For all concentrations, choline will be expressed as mg/100 g reconstituted liquids. Report as total choline.

Method Performance Requirements a for L-Carnitine (SMPR 2012.010)

Analytical range

0.16-20 b ≤0.16 b

Limit of quantitation (LOQ) Repeatability (RSD r )

0.16-20 b

≤8%

Recovery

90 to 110% of mean spiked recovery over the range of the assay

Reproducibility (RSD R ) ≤ 15% a Concentrations apply to: (1) “ready-to-feed” liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. b mg/100 g reconstituted final product. 0.16-20 b

- 19 -