AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2015.10 (Jan. 2019) Carn-07 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Time, min

Flow rate, mL/min

Phase B, %

0

1.00 1.00 1.00 1.00 1.00 1.00

0 0

1.0 1.5 2.5 3.0 4.2

100 100

0 0

Table 2015.10C. LCMS parameters LCMS Settings* LCMS model Sciex 4000

Sciex 6500

Ionization mode IonSpray voltage

MRM 1000V 550ºC

MRM 2000V 550ºC

Turbo IonSpray Temp Entrance Potential (EP

10V

10V

Collision Gas (CAD) Nitrogen, 5.0

Nitrogen, 5.0 Nitrogen, 20.0 Nitrogen, 60.0 Nitrogen, 60.0 Y = 5 mm, X = 5 mm

Curtain Gas (CUR)

Nitrogen, 20.0

Nebulizing Gas (GS1) Nitrogen, 60.0 Nebulizing Gas (GS2) Nitrogen, 60.0

Needle Position

Y = 5 mm, X = 5 mm

Agilent 6490

LCMS model Ionization mode

MRM 380V

Fragmentor

Cell Accelerator Gas Temperature

5V

200ºC

Gas Flow Nebulizer

20 L/min 30 psi 225 ºC 1500V 500V 200V 100V 11 L/min

Sheath Gas Temp Sheath Gas Flow Nozzel Voltage + High Pressure RF + Low Pressure RF + LCMS model Ionization mode Capillary Voltage RF lens voltage Source Temp Nebulizer Temp Nebulizer Gas: Desolvation Gas: Capillary + API Gas

Waters TQD

MRM

2000 V 0.1eV 150ºC 450ºC

On

Nitrogen

900 L/hr, Nitrogen 50 L/hr, Nitrogen 0.25 mL/min Argon

Cone Gas: Collision Gas:

API Gas

On

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