AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2015.10 (Jan. 2019) Carn-07 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Nebulizer Gas:

Nitrogen

Analyte

Transition use/type

Transition Monitored Q1/Q3** 162.0/103.0 165.0/103.0 162.0/84.4 162.0/59.1 104.2/60.0 104.2/42.2 108.2/60.0

Dwell time (msec)**

Collision Energy (V)**

Retention Time (min)

Carnitine Carnitine- d3 Carnitine Carnitine Choline Choline Choline-d4

Quantitation

100 100 100 100 100 100 100

25 25 29 27 25 25 25

1.7 1.7 1.7 1.7 2.1 2.1 2.1

Internal Standard Qualifier Qualifier

Quantitation Qualifier

Internal Standard

* The MS/MS settings may be modified except for ionization type and mode to obtain optimum chromatography and sensitivity. Exact mass ions may vary slightly from instrument to instrument because of unit resolution of quadrupole mass spectrometers. ** Settings listed are for Sciex series of LCMS. Precursor/product masses and instrument settings may vary.

References

[1] AOAC SMPR 2012.010, J.AOAC Int . 96 , 488(2013). [2] AOAC SMPR 2012.013, J.AOAC Int. 96 , 492(2013). [3] AOAC First Action Official Method 2012.17, J.AOAC Int. 96 , 1082(2013). [4] CARN-05, AOAC First Action Official Method 2014.04.

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