AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Experimental

A. Apparatus

(a) LC system . – Prominence (Shimadzu, Kyoto, Japan) or equivalent.

(b) MS/MS system . – API 4000 with Electrospray Ionization (ESI) (ABSciex, Framingham, MA)

or equivalent.

(c) Mass Spectrometry Software . – Analyst (ABSciex) or equivalent.

(d) Analytical column . – Zorbax 300-SCX, 3.0 x 50 mm, 5 µm (Agilent, Santa Clara, CA) or

equivalent.

(e) Microwave . – MARS6 (CEM, Mathews, NC) or equivalent.

(f) Microwave turntable, liner, and cap . – MARSXpress, 55 mL PFA Teflon®, 40 position

(CEM or equivalent).

(g) Vortex mixer. – (VWR, West Chester, PA) or equivalent.

(h) Analytical balances. – Model CPA225D (Sartorius, Goettingen, Germany) or equivalent.

(i) Horizontal shaker. – Model 6010 (Eberbach, Ann Arbor, MI) or equivalent.

(j) Magnetic stir plate. – Model PC-420D (Corning, Corning, NY) or equivalent.

(k) Positive displacement pipets. – Microman, various sizes (Gilson, Middleton, WI) or

equivalent.

(l) Repeater positive displacement pipet . – Repeater Plus (Eppendorf, Hamburg, Germany) or

equivalent.

(m) Polypropylene tubes . – Digitube, assorted sizes (SCP Science, Montreal, Canada) or

equivalent.

(n) Mobile phase containers . – 2 L glass (VWR) or equivalent.

(o) Syringe filters . – 0.45 µm PTFE and GHP (Pall, Plano, TX) or equivalent.