AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

(p) Disposable syringes . – 3 mL (BD Biosciences, Franklin Lakes, NJ) or equivalent.

(q) Graduated cylinders . – Assorted sizes (VWR) or equivalent.

(r) Magnetic stir bars . – 7.9 x 50 mm (VWR) or equivalent.

(s) Autosampler vials/caps . – 1.5 mL silanized crimp top (VWR) or equivalent.

(t) Microcentrifuge tubes . – 1.5 mL polypropylene (VWR) or equivalent.

(u) Bottle top dispenser . – 5 mL acid resistant (Brand, Essex, CT) or equivalent.

(v) Desiccator . – glass (VWR) or equivalent.

Note : Non-specific binding can occur with these analytes when using glassware, so plasticware

should be used at all times for standard/sample preparation. All laboratory plasticware should be

single-use whenever possible. Positive displacement pipets are also mandatory for pipeting to

avoid contamination and for accuracy with organic solvents.

B. Chemicals and Reagents

(a) Water . – Optima MS grade (Thermo Fisher Scientific, Waltham, MA) or equivalent.

(b) Acetonitrile . – Optima MS grade (Thermo Fisher Scientific) or equivalent.

(c) Ammonium formate . – Optima MS grade (Thermo Fisher Scientific) or equivalent.

(d) Formic acid . – Optima MS grade (Thermo Fisher Scientific) or equivalent.

(e) Nitric acid . – 70% w/w, ACS grade (Avantor, Center Valley, PA) or equivalent.

(f) Isopropanol . – Optima MS grade (Thermo Fisher Scientific) or equivalent.

(g) Desiccant . – (VWR) or equivalent.

(h) Reference Standard . – L-Carnitine (USP, Rockville, MD) or equivalent.

(i) Reference Standard . – Choline Bitartrate (TCI, Tokyo, Japan) or equivalent.

(j) Reference Internal Standard . – L-Carnitine-d3 HCl (CDN Isotopes, Pointe Claire, Québec,

Canada) or equivalent.