AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

weighting was used for both analytes. Confirmation was achieved through analysis of ion ratios

between samples and reference standards for at least one additional transition listed in the table.

The concentration of each analyte in a sample was calculated by the following equation:

where R = results expressed in mg/100g, C = concentration of the analyte in the injected solution

in ng/mL, V = volume of the initial extract in mL, S = sample weight in g, and D = dilution

factor, the inverse of any dilution made. All results were calculated on a ready to feed or

reconstituted basis of 25 g diluted to 225 g with water, except for SRM 1849a that was calculated

back to powder basis.

Results and Discussion

Linearity was assessed from the percent deviation from theoretical concentration across

the working standard range (Table 3). The results from the validation showed an overall

repeatability for free and total choline of 1.9 and 2.3 %RSD r

, while the overall intermediate

precision obtained for free and total choline was 2.4 and 2.7 %RSD INT

, respectively (Table 4).

Free and total carnitine had an overall repeatability of 2.9 and 2.7 %RSDr, while the overall

intermediate precision obtained for free and total carnitine was 3.3 and 3.1 %RSD INT ,

respectively (Table 5). The average results obtained from the analysis of NIST SRM 1849a were

within the certified ranges or close to the information mass fraction value. Only an information

mass fraction value is given for free choline, while there is currently nothing provided for total

carnitine from NIST. The average total recoveries (endogenous + added) shown in Tables 6 and

7 for all matrices tested from LOQ to the upper ranges required in the SMPR were 95.9 –

103.6%. Analysis of bound sources of carnitine and choline analyzed in duplicate over three

days gave average recoveries of 104.6% for acetylcholine, 96.7% for phosphatidylcholine, and