AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

Chol-08/Carn-07 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 2. Method Performance Requirements for Total Choline (SMPR 2012.013)

2-250 a

Analytical range

0.7 a 2.0 a

Limit of detection (LOD) Limit of quantitation Repeatability (RSD r )

2 a

≤10% ≤5%

20 a 100 a 200 a

Recovery

90 to 110% of mean spiked recovery over the range of the assay

2 a

Reproducibility (RSD R )

≤15% ≤10%

20 a 100 a 200 a

a mg/100 g Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. For all concentrations, choline will be expressed as mg/100 g reconstituted liquids. Report as total choline.

Table 3. Method Performance Requirements for L-Carnitine (SMPR 2012.010)

0.16-20 b ≤0.16 b

Analytical range

Limit of quantitation (LOQ) Repeatability (RSD r )

0.16-20 b

≤8%

Recovery

90 to 110% of mean spiked recovery over the range of the assay

Reproducibility (RSD R ) ≤ 15% a Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3) liquid concentrates diluted 1:1 by weight. b mg/100 g reconstituted final product. 0.16-20 b