BCRB 2018

19/09/2018

Clonogenic cell survival: ex vivo

cell suspension

tumor

X

culture dish

Mouse with tumor

colonies after in vitro growth

X

CON

Ex-vivo assays

• Culturing as organoids • Tumors are excised, reduced to single cells, and grown in 3D matrix • Measurement of tumor stem cells • Show potential to differentiate in all tumor subtype cells • Lack of environmental factors and vascularisation

22