AOAC SPIFAN Stakeholder Panel (August 25, 2018)

BIS Analytical Method for B12 IS 7529: 1975 RA 2005 – Method for estimation of vitamin B12 in food stuff - Based on Microbiological method For very low levels of B12 in a product, BIS says - ‘The Indian Standards indicated are given for guidance only as they are under revision at present. As there is no other suitable and easily workable method at present, the manufacturers would be required to maintain records showing the quantity of the 'added vitamins', added to each batch .’ Microbiological Method for B12 Principle - 1. It relies on the specific requirement for vitamin B12 by certain bacterial organisms (e.g. Lactobacillus delbreueckii ) to enable their growth in a supporting medium. 2. Under appropriate conditions, the amount of growth obtained is proportional to the amount of vitamin B12 in a test extract. 3. The turbidity obtained is measured using a spectrophotometer