AOAC SPIFAN Stakeholder Panel (August 25, 2018)

AOAC methods AOAC 2011.16 Determination of vitamin B12 in infant formula and adult nutritionals by surface plasmon resonance: (test kit method) – Biacore technology (First action) AOAC 2011.10 Determination of vitamin B12 in infant formula and adult nutritionals by HPLC: (Final Action) – HPLC with SPE clean-up (column switching)

CEN / BS: There are no approved methods for vitamin B12 in infant/adult nutrition products

Analytical Method for B12 Why AOAC 2011.10? 9 SLV – 12 products (including NIST SRM, placebo and 10 other milk based products) 9 Comparison of HPLC results with microbiological method 9 Inter-laboratory Study • 2014 – Final action method • 2015 – ISO 20634 • 2016 – Voted as Dispute resolution method by AOAC, ISO and IDF