AOAC SPIFAN Stakeholder Panel (August 25, 2018)

SLV Protocol Based on AOAC Guidelines for SLV of Chemical Methods for Dietary Supplements and Botanicals A. Linearity - 6 point calibration curve in triplicate, range – 2.5 to 25 μg/ L B. Precision - Every matrix in triplicate, 5 days, 2 analysts C. Accuracy - 50% and 100% spike (of its mean precision value), Study conducted on 3 days by 2 analysts. The Journey of SLV Study ™ The author of the AOAC method – Ms. Karen Schimpf from Abbott, USA, guided us at various occasions during the entire single lab validation study – right from verifying the SLV protocol to validation of results generated in the study ™ Abbott laboratory at Bangalore – This method was already validated and was under their scope of accreditation. They supported the study by providing training to the Envirocare analyst