AOAC SPIFAN Stakeholder Panel (August 25, 2018)

Method Optimization – Malt Products

Attempts were made to reduce the matrix interference

1. Reducing sample weight 2. Reducing the volume loaded on the SPE cartridge 3. Improving the resolution by further delaying the gradient

However, none of these improved the chromatography

Method Optimization – Malt Products

• The challenge was then shared with the author and she suggested modifications in the sample preparation. • These were first evaluated at Abbott’s Bangalore lab • Abbott’s analyst then visited Envirocare and imparted training on these modifications.