AOAC SPIFAN Stakeholder Panel (August 25, 2018)

WG of infant formula & dairy comments so far

Type A e.g: Standard Standard

Tracking evaluation opinion Tracking evaluation opinion

Suggested conclusion Suggested conclusion

GB 29989-2013 GB 29989-2013

Adding LC-MS/MS method Adding LC-MS/MS method

turn down turn down

"3.1.4 chromogenic agent" 2-nitrobenzoic acid (C14H8N2O8S2) should be modified to "5,5-dithiobis (2-nitrobenzoic acid) (C14H8N2O8S2) In order to meet the requirements of GB10765 and GB1076 for the equivalent value of alpha-tocopherol, it is suggested that the formula for calculating the equivalent value of alpha-tocopherol should be clearly defined in the method Increasing the use of phosphatase during sample preparation for the detection of riboflavin (riboflavin-5-sodium phosphate) in phosphoric acid form Determination of nitrite in infant formula rice flour, sample preparation, before the step of removing fat and protein, amylase for enzymatic hydrolysis "3.1.4 chromogenic agent" 2-nitrobenzoic acid (C14H8N2O8S2) should be modified to "5,5-dithiobis (2-nitrobenzoic acid) (C14H8N2O8S2) In order to meet the requirements of GB10765 and GB1076 for the equivalent value of alpha-tocopherol, it is suggested that the formula for calculating the equivalent value of alpha-tocopherol should be clearly defined in the method Increasing the use of phosphatase during sample preparation for the detection of riboflavin (riboflavin-5-sodium phosphate) in phosphoric acid form Determination of nitrite in infant formula rice flour, sample preparation, before the step of removing fat and protein, amylase for enzymatic hydrolysis

GB 29989-2013 GB 29989-2013

accept accept

GB 5009.82-2016 GB 5009.82-2016

accept accept

GB 5009.85-2016 GB 5009.85-2016

accept accept

GB 5009.33-2016 GB 5009.33-2016

accept accept

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WG of infant formula & dairy comments so far

Type B e.g:

Standard

Comments collected

estimated method performance characteristics

GB 5009.33-2016

The recovery rate of nitrite in infant formula milk powder is low.

recovery (Accuracy)

Fat values are often low when making cereal supplements for infants and young children. Ammonia water bath time is extended from 15 to 20 minutes to 40 minutes during the starch sample preparation. To ensure complete hydrolysis of fat. The detection limit and quantitation limit can not reach the level specified by the standard.

GB 5009.6-2016

Accuracy

GB 5009.24-2016

Limit of Detection; Limit of Quantitation

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