AOAC ISPAM Stakeholder Panel Meeting Book (August 26, 2018)

Priority Sequence – AOAC Mid-Year Meeting 2018

1. Number of out of range levels per assay a. Recommendations for dealing with discordant results 2. Difference of Means (Acceptance criteria) a. Indicator organism vs. pathogens b. Principle of the technology

3. Determine acceptance criteria for low inoculation level replicates

Data Research on Discrepant Results

Methods

Total Viable/ Aerobic Count

Yeast and Mold

Total # of Methods Reviewed Total # of Matrices Evaluated Total # Levels of Contamination

8

5

100

87

400

350

OOS (DM > 0.5)

14 (3.5%)

25 (7.1%)

2 – Low 9 – Medium 3- High

5- low 12 – Medium 8 – High

Contamination Level OOS

Dairy Matrices (Milk, Yogurt) Low Moisture Foods (Flour Tortilla, Rice Cakes)

Stainless Steel Sponges, Raw Meats/Fish Skim Milk

Example of Matrices OOS