Cannabis ERP

Cannabis ERP Method Reviews and Decisions

AOAC Method #

Manuscript Title, Submitter and Reviewers

ERP Decisions / Consensus

Title: Quantitation of Cannabinoids in Cannabis Dried Plant Materials and Concentrates Using Liquid Chromatography- Diode Array Detection Technique with Optional Mass Spectrometric Detection Author(s): Lukas Vaclavik, Frantisek Benes, Ales Krmela, Veronika Svobodova, Jana Hajslova and Katerina Mastovska

MOTION to move this method to First Action Official Methods of Analysis Status (Rimmer/Audino) 10 in favor, 2 opposed, 1 abstentions. The motion failed.

Reasoning of Dissenters:

Indick: The SMPRs clearly call for Limit of Quantitation (LOQ) and recovery data and neither are clearly provided. Further, he had concerns about the extraction and processing. Yadlapalli: Agreed, LOQ and recovery data is needed.

Submitted by: Katerina Mastovska, Covance

After further discussion, the ERP agreed that more data is required. ____________________________________________________________ First Action Requirements:

Primary Reviewer: Pete Indick, Microbac Secondary Reviewers: Mike Schaffer, Psychemedics and Christopher Hudalla, Waters

• Processing information o Ideal bulk sample size recommendations o Grinding size • Spiked recovery o Spike addition (low level plant acceptable)

CAN-001

o Well characterized standard mixture used for spiked recovery. o Required compounds: CBDA, THC o Levels: LOQ (Low and Mid Range, as per SMPR, on the compounds listed above) • Present LOQ data in matrix data as a consolidated table for all compounds. MOTION to accept the First Action Official Methods recommendations as presented (Sweeney / Johnson). 13 in favor, 0 opposed, 1 abstention. The motion passed.

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