AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)
2017.03 (JAN 2019) Amino-02-(ERP Review for Pubs) Tryptophan by enz hyd_Abbott Labs_Method_10172018
FOR ERP USE ONLY DO NOT DISTRIBUTE
HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories
For Blank:
Blank mg = 0.1154 – (-0.004522) × 0.0403 L 0.007082
Tryptophan in Blank = 0.6824 mg/L
Tryptophan Value in g/100g of sample:
TRP (g/100 g sample) = (CTS-CTB) × (Volume/Sample Weight (g)) ×
100/1000
Where:
CTS = Concentration of Tryptophan in Sample CTB = Concentration of Tryptophan in Blank Volume = Final volume in Liters i.e. 50 mL = 0.05L
Sample Computation for RTF Sample: TRP (g/100g Sample) = (6.125 mg/L – 0.6824 mg/L) × (0.05L / 1.0095g) × 100/1000
Tryptophan in g/100g of sample = 0.0270 g/100g sample
Conversion to Protein Basis:
= TRP g 100 100 g protein 100 g Sample % Protein ×
TRP g
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