AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)
2017.03 (JAN 2019) Amino-02-(ERP Review for Pubs) Tryptophan by enz hyd_Abbott Labs_Method_10172018
FOR ERP USE ONLY DO NOT DISTRIBUTE
HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories
FIGURE 2 Typical Blank Chromatogram
0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50
5MDLT - 6.182
LU
TRP_SA - 2.489
7.50
8.00
8.50
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
6.50
7.00
Minutes
FIGURE 3 Typical Sample Chromatogram (NIST Standard Reference Material 1849a)
12.00
11.00
10.00
9.00
TRP_SA - 2.483
5MDLT - 6.175
8.00
7.00
6.00
LU
5.00
4.00
3.00
2.00
1.00
3.106
6.875
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
6.50
7.00
7.50
8.00
8.50
Minutes
Page
16
Made with FlippingBook - Online Brochure Maker