AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method

FOR ERP USE ONLY DO NOT DISTRIBUTE

HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis

Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories

c) Volumetric pipettes: 0.5, 1, 2, 5, 10 mL d) 50 mL polypropylene centrifuge tubes e) Rack capable of holding tubes from d) f) Volumetric flasks, 50 mL, 100 mL, 500 mL, 2000 mL g) pH meter h) Constant temperature oven capable of maintaining 50 (+/- 1) degree Centigrade i) Thermometer (-20 to 110°C), calibrated j) Vortex mixer k) Sonic water bath l) Stir bars m) Stir plates n) ACRODISC disposable filter assembly, 0.45 micron pore size From Gelman Scientific, cat. # 4217, or equivalent o) 3 cc or 5 cc disposable syringe (s) p) Weighing paper q) Weigh boats r) Funnel(s) s) Water bottle t) Membrane filter, 0.45 micron u) Autosampler vials and caps (see autosampler requirements). a)v) Analytical Column: Preferred column: YMC PACPACK C8 50 × 3 mm column with, 3 micronum particle size – Part #, 3.0 50 mm – YMC America, INC, Product No. OC12S03- 053030 –0503WT, or equivalent.alternate b)w) HPLC system with autosampler, column oven to maintain 30°C, Fluorescence Detector, binary or quaternary HPLC Pump with degasser, HPLC peak area integrator or chromatography data processor.

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