AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method

FOR ERP USE ONLY DO NOT DISTRIBUTE

HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis

Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories

I . METHOD PERFORMANCE (Data from AMINO-02 AOAC SLV) 1. Accuracy:

Table 1

Accuracy - Spike Recovery

Spike Recovery

Product

SPIFAN Code

Analyte

Nr. replicates (n) Native level

+ Spike Level 1

+ Spike Level 2

Avg (%) RSD % Avg (%) RSD (%)

SRM1849a

CLC10-B

TRP TRP TRP TRP TRP TRP TRP TRP

12 12 11 12 10 12 12 12

20.1 25.3 18.3 21.4 32.2 19.5 18.3 91.3

99.9

1.8 3.3 8.6 5.2 2.5 3.0 5.1 1.6

100.9 104.1 101.5 102.4 101.7 99.5

2.4 2.7 4.8 3.9 2.7 2.0 3.5 1.3

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z 00859RF00 00795RF K16NTAV E10NWZC 00729RF00

104.3 103.7 100.7 100.5 96.2

Adult Nutritional Powder Low Fat

Infant Elemental Powder

Infant Formula Powder Milk Based Infant Formula Powder Soy Based Adult Nutritional RTF High Fat

93.8

99.5

104.8

104.9

Global Mean % 100.5

3.9 101.8

2.9

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