AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)
2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method
FOR ERP USE ONLY DO NOT DISTRIBUTE
HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis
Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories
HJ . FIGURES FIGURE 1 Typical 5/50 Standard Chromatogram
13.00
12.00
11.00
10.00
TRP_SA - 2.486
9.00
5MDLT - 6.184
8.00
7.00
LU
6.00
5.00
4.00
3.00
2.00
1.00
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
6.50
7.00
7.50
8.00
8.50
Minutes
13.00
12.00
11.00
10.00
TRP_SA - 2.486
9.00
5MDLT - 6.184
8.00
7.00
LU
6.00
5.00
4.00
3.00
2.00
1.00
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
6.50
7.00
7.50
8.00
8.50
Minutes
Page
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