ClearLLab 10C Case Book

CHRONIC LYMPHOCYTIC LEUKEMIA/SMALL LYMPHOCYTIC LYMPHOMA Case #7: Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

Clinical Vignette

This 65-year-old male presents with lymphocytosis. A peripheral whole blood sample is submitted for flow cytometric immunophenotyping using ClearLLab 10C Panels.

Flow Cytometric Immunophenotyping

B Cell Tube

Figure 1: This Time vs CD45 dot plot is ungated and shows all events collected sequentially. This plot is intended to evaluate for fluidic perturbation during sample acquisition. Stable acquisition is represented by a uniform pattern of events over time. Events that deviate from the stable pattern can be excluded in the Time gate.

Figure 2: This FS INT vs FS PEAK dot plot shows events in the Time gate. This plot is intended to exclude cell doublets or aggregates. Singlet events show a linear relationship for INT vs PEAK and are included in the Singlets gate, while doublets lie outside the linear relationship.

Table of Contents > Neoplastic Process of B-cell Origin > Case #7: Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

Every Event Matters

154 Beckman Coulter • ClearLLab 10C Panels • C30134 AA