ClearLLab 10C Case Book

B Cell Tube

Figure 7: This Kappa vs Side Scatter dot plot shows all viable cells. Surface kappa light chain is expressed onmature B cells (orange) and late stage immature B cells. The proportion of kappa positive B cells is normally greater than kappa negative (lambda positive) B cells. Apparent kappa positivity is seen onmonocytes (green) due to Fc receptor-mediated binding of immunoglobulin. The majority of the CD19 positive cells (orange) lack kappa light chain expression.

Figure 8: This Lambda vs Side Scatter dot plot shows all viable cells. Surface lambda light chain is expressed onmature B cells (orange) and late stage immature B cells. The proportion of lambda positive B cells is normally less than lambda negative (kappa positive) B cells. Apparent lambda positivity is seen onmonocytes (green) due to Fc receptor-mediated binding of immunoglobulin. The majority of the CD19 positive cells (orange) have surface lambda light chain expression.

Figure 9: This CD10 vs Side Scatter dot plot shows all viable cells. CD10 is expressed on immature B cells (purple), mature germinal center B cells, andmature granulocytes(blue).Thegranulocyteshavehighsidescatter, incontrastto lymphoid cells that have low side scatter. The CD19 positive population (orange) is negative for CD10. The apparent CD10 positivity on the aberrant population (orange) is likely due to bright lambda light chain causing an increase in ECD background.

Figure 10: This CD5 vs Side Scatter dot plot shows all viable cells. CD5 is expressed on mature and immature T cells (red), as well as dimly a subset of mature B cells. These lymphoid cells typically have low side scatter. The CD19 positive population (orange) expresses CD5 at a slightly lower level than that seen on T cells (red).

Table of Contents > Neoplastic Process of B-cell Origin > Case #8: Mantle cell lymphoma

Every Event Matters

180 Beckman Coulter • ClearLLab 10C Panels • C30134 AA