ClearLLab 10C Case Book

M1 Cell Tube

Figure 9: This CD13 vs Side Scatter dot plot shows all viable cells. CD13 is expressed on granulocytes, monocytes, and myeloid progenitors. Lymphocytes (red) are largely negative for CD13.

Figure10:ThisCD64vsSideScatterdotplotshowsallviablecells.CD64 isexpressed at its highest level on mature and immature monocytes and at a slightly lower level on promyelocytes and myelocytes. CD64 is not well expressed on resting mature granulocytes, but increases in expression with granulocyte activation. Lymphocytes (red) are negative for CD64.

Figure 11: This CD34 vs Side Scatter dot plot shows all viable cells. CD34 is a marker of early hematopoietic progenitors. It is expressed on hematopoietic stem cells, early myeloid progenitors (myeloblasts), and immature B and T cells (lymphoblasts). Mature lymphocytes (red) are negative for CD34. Tissues rarely contain CD34 positive progenitors.

Figure 12: This CD14 vs Side Scatter dot plot shows all viable cells. CD14 is expressed at a high level on mature monocytes, at a variably lower level on immature monocytes, and at a low level onmature granulocytes. Mature lymphocytes (red) are negative for CD14.

Table of Contents > No Immunophenotypic Abnormality > Case #3: Normal Lymph Node

Every Event Matters

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Beckman Coulter • ClearLLab 10C Panels • C30134 AA