ClearLLab 10C Case Book

NEOPLASTIC PROCESS OF B-CELL ORIGIN B cell neoplasms, which comprise the majority of all lymphoid neoplasms, are a diverse group of tumors that include acute lymphoblastic leukemias/lymphomas and mature B cell leukemias/lymphomas. To varying degrees, these neoplasms recapitulate normal stages of B cell differentiation and typically have distinctive immunophenotypes that permit classification according to their postulated cell of origin. In addition, cytogenetic profiles, genotype, and immunophenotype of the malignant cell have had considerable impact on prognostic and therapeutic stratifications of patients with B cell neoplasms.

B LYMPHOBLASTIC LEUKEMIA/LYMPHOBLASTIC LYMPHOMA Case #4: B Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma

Clinical Vignette

This 70-year-oldmale presents with circulating blasts. A peripheral whole blood sample is submitted for flow cytometric immunophenotyping using ClearLLab 10C Panels.

Flow Cytometric Immunophenotyping

B Cell Tube

Figure 1: This Time vs CD45 dot plot is ungated and shows all events collected sequentially. This plot is intended to evaluate for fluidic perturbation during sample acquisition. Stable acquisition is represented by a uniform pattern of events over time. Events that deviate from the stable pattern can be excluded in the Time gate.

Figure 2: This FS INT vs FS PEAK dot plot shows events in the Time gate. This plot is intended to exclude cell doublets or aggregates. Singlet events show a linear relationship for INT vs PEAK and are included in the Singlets gate, while doublets lie outside the linear relationship.

Table of Contents > Neoplastic Process of B-cell Origin > Case #4: B Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma

Every Event Matters

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Beckman Coulter • ClearLLab 10C Panels • C30134 AA