AOAC SPSFAM March 16 2015 eBook

DRAFT AOAC SMPR 2015.XXX; Version 6; February 27, 2015 1 2 Standard Method Performance Requirements for 3 Quantitation of Arsenic Species in Selected Foods and Beverages 4 5 Intended Use: Reference method for dispute resolution.

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1 Purpose 8 9 AOAC Standard Method Performance Requirements SM SMPR’s describe the minimum 10 recommended performance characteristics to be used during the evaluation of a method. The 11 evaluation may be an on-site verification, a single-laboratory validation, or a multi-site 12 collaborative study. SMPRs are written and adopted by AOAC Stakeholder Panels composed of 13 representatives from the industry, regulatory organizations, contract laboratories, test kit 14 manufacturers, and academic institutions. AOAC SMPRs are used by AOAC Expert Review 15 Panels in their evaluation of validation study data for method being considered for Performance 16 Tested Methods or AOAC Official Methods of Analysis , and can be used as acceptance criteria for 17 verification at user laboratories. [Refer to Appendix F: Guidelines for Standard Method 18 Performance Requirements , Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th 19 Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA.] 22 23 Quantitation of arsenic (As) species and/or total inorganic arsenic in selected food and 24 beverage matrixes (see table I for a list of selected foods.) 27 28 Separation of species by either ion chromatography (IC or high-performance liquid 29 chromatography (HPLC and detection of separated species by inductively coupled plasma-mass 30 spectrometry (ICP-MS). 33 34 Arsenic Species .— For the purposes of this SMPR arsenic species includes the compounds listed 35 in table 2. 36 37 Limit of quantitation (LOQ) .—The minimum concentration or mass of analyte in a given matrix 38 that can be reported as a quantitative result. 39 40 Quantitative method. —Method of analysis which response is the amount of the analyte 41 measured either directly (enumeration in a mass or a volume), or indirectly (color, absorbance, 42 impedance, etc.) in a certain amount of sample. 43 44 Repeatability .—Variation arising when all efforts are made to keep conditions constant by using 45 the same instrument and operator and repeating during a short time period. Expressed as the 46 repeatability standard deviation (SDr); or % repeatability relative standard deviation (%RSDr). 20 21 2 Applicability 25 26 3 Preferred Analytical Technique 31 32 4 Definitions

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