AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

6XUMXKYY OT

Conclusions/proposals and feedback

‹ Internal standard D3-d6 is better than D3-d3: Yes, D3-d6 is better, but need more data to compare the difference. ‹ ISO 14892:2002 method of sample re-dissolution makes the method more durable. Adopted by GB drafter. ‹ PTAD derivatization used in OM incérasse specificity and selectivity, submited to CFSA. ‹ Refer to AOAC standard, GB 5009.82 will be divided into 2 standards to test Vit A&E, and Vit D separately

6XUMXKYY OT

First round Methods: GB 5009.82-2016 Method 3, Method 4 ˗ ISO 14892:2002 |IDF 177:2002 Conclusion: T test, No difference between these 3 methods.

Two-way ANOVA, No difference between these 3 methods. Significant differences between different labs.

Two-way ANOVA (SPSS 20)

III රᒣᯩ઼ Type Ϫ SS

df

൷ᯩ mean square

F

Sig.

Ⓚ

ṑ↓⁑ර Calibration model

33.291 a

7 1 5 2

4.756

6.681

.000 .000 .000 .191

ᡚ䐍 Intercept

7023.486

7023.486

9866.020

ᇎ傼ᇔ Lab

30.894

6.179 1.198

8.680 1.683

ḷ߶ᯩ⌅ Stannard methods

2.397

䈟ᐞ Error ᙫ䇑 Total

71.189

100 108 107

.712

7127.966 104.480

ṑ↓Ⲵᙫ䇑 Total calibration