AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

8. Accuracy (Trueness)

X Paragraph with conditions for analysis of a CRM or Reference Material X Added: “Min. 9 independent replicates on each of 3 days” X Added: “A t-test or other statistical test should be used to evaluate method bias” X Paragraph on spike recovery was updated due to lack of SPIFAN kit. Study Directors may agree on the samples to be included. X Fortification levels: For unfortified matrices, use spike levels of 50, 100 and 150% of typical fortified target. For fortified matrices, use fortification levels of 0%, +50% and +100% of the fortified range. X Matrices used for estimating recoveries may or may not coincide with one or more of those selected for precision studies. If there is overlap, then a single 2 x 6 replication of the unspiked matrix covers both requirements for that sample type. X Comparison to reference methods.— This is not required as matter of routine, because the additional effort and lack of appropriate reference methods. SDs may choose to collect reference method comparison data. X Paragraph deleted with 2 topics, redundant:

Next steps

X Today: Review proposed changes by stakeholders X Next: Review by ERP members X Final review by OMB