AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

Working group activities

Discussed during the calls • Working Group had experience and answers to questions because of the 2’FL and LNnT SMPRs : e.g. selectivity/interferences; (non available) reference materials • The “main body text” of the 2’FL and LNnT – approved – SMPRs kept as template • Emphasis was given to: Analytical range per HMO Æ mainly based on data for breast milk

September 10, 2020 • All 5 SMPRs presented for voting Æ not enough votes received in 2020

Specific Performance Claims: 3’-SL and 6’-SL

Table 1: Method Performance Requirements a

3’-sialyllactose (3’-SL) 1.5 – 150 mg/100g

6’-sialyllactose (6’-SL) 2 – 150 mg/100g

Analytical range Limit of Quantitation (LOQ)

ч 1.2 mg/100g

ч 1.6 mg/100g 85-110% (2-20 mg/100g) 90-110% (>20mg/100g)

85-110% (1.5-20 mg/100g ) 90-110% (>20mg/100g)

Recovery

Repeatability (% RSD r ) Reproducibility (% RSD R )

ч ϱй

ч ϱй

ч ϭϬй ч ϭϬй a Concentrations apply to: i) “ready-to-feed’ liquids “as is”; ii) reconstituted powders (25 g into 200 g of water); and iii) liquid concentrates diluted 1:1 by weight.