AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

Specific Performance Claims: 3-FL and DFL

Table 1: Method Performance Requirements a

3-Fucosyllactose (3-FL) 4 – 600 mg/100g

Difucosyllactose (DFL) 1.5 – 100 mg/100g

Analytical range

Limit of Quantitation (LOQ)

ч 1.2 mg/100g

ч 3.2 mg/100g 85-110% (4-20 mg/100g ) 90-110% (>20mg/100g)

85-110% (1.5-20 mg/100g ) 90-110% (>20mg/100g)

Recovery

Repeatability (% RSD r ) Reproducibility (% RSD R )

ч ϱй

ч ϱй

ч ϭϬй ч ϭϬй a Concentrations apply to: i) “ready-to-feed’ liquids “as is”; ii) reconstituted powders (25 g into 200 g of water); and iii) liquid concentrates diluted 1:1 by weight.

Specific Performance Claims: LNT Table 1: Method Performance Requirements a Analytical range 2 – 300 mg/100g Limit of Quantitation (LOQ) ч 1.6 mg/100g

85-110% (2-20 mg/100g ) 90-110% (>20mg/100g)

Recovery

Repeatability (% RSD r )

ч ϱй

Reproducibility (% RSD R ) ч ϭϬй a Concentrations apply to: i) “ready-to-feed’ liquids “as is”; ii) reconstituted powders (25 g into 200 g of water); and iii) liquid concentrates diluted 1:1 by weight.