AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

Beta-casein Peptide by Trypsin Digestion

Variants Peptide 49-97

A2-type IHPFAQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFLQPEVMGVSK

H1

IHPFAQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFIQPEVMGVSK

H2

IHPFAQTQSLVYPFPGPIPNSLPENIPPLTQTPVVVPPFLQPEVLGVSK

IHPAFQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFLQPEVLGVSK

I

A1-type IHPFAQTQSLVYPFPGPIHNSLPQNIPPLTQTPVVVPPFLQPEVMGVSK

De Poi R. et al. (2020) Food Analytical Methods 13:2177–2187

Beta-casein Variants Natural variants

Amino acid change

A2-type A3

H106Q Sp18K

D E

E36K

H1 H2

R25C; L88I; L137P; P138L

Q72E; M93L; P138E

I

M93L

A1-type A1

P67H; E117Q; L137P; P138L; Q175E

B C F G

P67H; S122R

Sp35S; E37K; P67H

P67H; P152L

P67H; E117Q; P138L; Q175E

Farrell, H. M. et al. (2004) J. Dairy Sci. 87:1641–1674 De Poi R. et al. (2020) Food Analytical Methods 13:2177–2187 https://www.uniprot.org/uniprot/P02666