AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

• Folch extraction (chloroform:methanol 2:1) is commonly used for phospholipids • Variations on Folch extraction, Bligh and Dyer methods, modified Röse-Gottlieb extraction also used • Strategies based on extraction of polar lipids fraction in to polar organic solvents • Disadvantage is the toxicity of chloroform Liquid-Liquid Extraction Sample Preparation

Sample Preparation

Solid Phase Extraction

• SPE using silica, or cyanopropyl, or aminopropyl modified silica used • After elimination of neutral lipids, organic solvents such as methanol or chloroform used to elute phospholipids • SPE used after liquid-liquid extraction to remove interferences and concentrate the phospholipids