AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

Analytical Techniques

Chromatography

• TLC, GC, HPLC all used for phospholipid analysis • RPLC commonly used to separate individual molecular species of a given class • In NPLC phospholipids elute based on the polarity of the head group • HILIC separation based on polar head group, also offers benefit of solvents compatibility with ESI-MS

Analytical Techniques

Detection

• Wide range of detectors used for phospholipids analysis • RI detectors are not particularly sensitive and response variabile with changes in temperature, solvent, and flow-rate • ELSD response dependent on phospholipid mass, compatible with gradients, stable to temperature variations, however non-linearity common