2019 Year 12 IB Extended Essays

3 Results

3.1 Raw Data

See appendix 1 (Raw data)

3.2 Processed Data

To determine the level of expression of a transcription factor in a cardiomyocyte, the number of other tissues that have similar or higher levels of expression will be counted. Each transcription factor will fall under the category of one of three levels of specificity. Transcription factors with high specificity will have 0 to 5 other tissue types Transcription factors with medium specificity will have 6 to 12 tissue types Transcription factors with low specificity will have 13 or more tissue types Table 3: Combined research results from three databases: Amazonia!, NCBI Gene Database, KEGG Pathway Database Target cell type Important Transcription factors Specificity to target cell type Known functions

Cardio myocytes Cardio myocytes

Baf60c

High (5)

 Regulate the transcription of certain genes by altering chromatin structure  Involved in the maturity of onset diabetes in the young.  Myocardial differentiation and function  Mutation associated with cardiac septal defects  Cellular senescence, thyroid hormone signalling pathways and in the development of tight junctions  Play a role in developing ventricular chambers, cardiac morphogenesis, limb and branchial arch development  Formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease  Interact with SRF and modulate cardiac development  Mice experiment shown requirement for embryonic cardiovascular development

Gata4

High (4)

Cardio myocytes

Hand2

Low (13)

Cardio myocytes Cardio myocytes Cardio myocytes

Hopx

Low (13+)

Hrt2

Medium (8)

Isl1

Low (13+)

 Involved in regulating insulin gene expression  Regulating the pluripotency of stem cells

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