SPDS Aloe Vera Method Book
OFFICIAL DOCUMENT
Date Issued: Supersedes:
Page:
8 of 15
Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR
N/A
Effective Date:
TBD
SOP #:
Revision #:
00
Prepared By: Department: Applies To:
Isaac Lee Quality Herbalife QC Laboratories
method requires the presence of all the peaks for glucose, malic acid and Acetylated glucomannan while Acacia gum NMR spectrum is missing glucose and malic acid peaks. In addition, the spectrum of Acacia gum contains distinguishable peaks around at 1.26ppm region, so any aloe vera sample diluted with the acacia gum is detectable (see figure 5). This ID method can screen out this adulterant for aloe vera ID and provide reliable conclusion for identification.
Figure 4. 1 H NMR spectrum of Acacia gum
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