SPDS Aloe Vera Method Book
OFFICIAL DOCUMENT
Date Issued: Supersedes:
Page:
9 of 15
Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR
N/A
Effective Date:
TBD
SOP #:
Revision #:
00
Prepared By: Department: Applies To:
Isaac Lee Quality Herbalife QC Laboratories
Figure 5. Comparison of 1 H NMR spectra of Acacia gum and Aloe vera samples Locust bean gum 1 H NMR spectrum of Locust bean gum showed no Glucose (α- at 5.2 ppm and β- at 4.6 ppm), Malic acid (4.3 ppm), and Acetylated glucomannan (2.0-2.3 ppm) peaks (Figure 6). Therefore, this material will be confirmed as not an aloe vera material. In addition, the spectrum of Locust bean gum contains distinguishable peaks around at 1.06ppm region, so any aloe vera sample diluted with the locust bean gum is detectable (see figure 7). Therefore, this proton NMR ID method gives correct and reliable conclusion.
For Use Only By Herbalife International. This document contains confidential and proprietary information belonging to Herbalife International - It must not be reproduced or disclosed to others without prior written approval
Made with FlippingBook - professional solution for displaying marketing and sales documents online