SPDS Aloe Vera Method Book
Date Issued: Supersedes:
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Procedure Title: Determination of Absolute Molecular Weight and Content of Polysaccharides in Aloe vera Fresh Leaf and Concentrated Juice Powder by SEC-MALS-RI
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Effective Date:
QCL
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Prepared By: Yuehong Zhang, Zhichao Bao Department: R&D Applies To: Herbalife International
The concentration of polysaccharides is determined by dividing resulting concentration obtained from RI detector over the injection mass calculated from ASTRA software.
Calculated mass (µg/100µl) x 10 x D.F.(mL) Sample Wt. (mg)
Polysaccharides (μg/mg) =
Calculated mass (µg/100µl) is known from Wyatt ASTRA 6.1.5.22 software D.F. = Dilution factor for sample
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DEFINITIONS N/A REFERENCES N/A
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FORMS / CHROMATOGRAMS 4.1 Chromatogram: Overlay light scattering (LS) and refractive index (RI) chromatograms of Aloe polysaccharides.
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OWNERSHIP/MANAGEMENT SIGN-OFF Approvals Refer to
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REVISION HISTORY Revision Date
Revised By
Summary of Items Revised New Test Method
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