2018 August 26 SPSFAM Book
AOAC Official Methods for Mycotoxin Analysis
29 AOAC official methods for aflatoxins 7 AOAC official methods for ochratoxin A 3 AOAC official methods for fumonisins 2 AOAC official methods for deoxynivalenol 3 AOAC official methods for zearalenone
# Publications
Year (1970-2018)
Source: http://eoma.aoac.org/
Source: www.pubmed.gov
Analysis of Aflatoxin M 1 in Milk FDA/UMD IFSTL Mycotoxin Training Course (College Park, Dec. 2013)
Candidate methods for the analysis of aflatoxin M 1
in milk
Procedure 1 (provided by the PT organizer)
Procedure 2 (FDA/CFSAN)
AOAC 2000.08 method
0.5 g milk + [ 13 C]-aflatoxin M 1
50 mL milk Immunoaffinity column Wash the column (20 mL H 2 O) Elute column (4 mL ACN) Evaporate eluent to dryness Reconstitute
2 mL milk + 2 mL ACN
Vortex 30s/sonicate 30min Centrifuge 10 min at 4000 rpm 2 mL top layer + 0.5 g NaCl Vortex 30s/sonicate 30min 0.6 mL top layer/freeze out 1 hr Centrifuge at 2 o C at 15,000 rpm Dilute filtrate 10x
Add 4.5 mL acetonitrile/water (50/50)
Hand shake 2 min
Centrifuge 15 min at 4500 rpm
LC-Fluorescence
(LC-MS for confirmation)
LC-MS
LC-MS
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