2018 August 26 SPSFAM Book

Analytical Methods : Lactose

• Polarimetric methods (AOAC 896.01- lactose in milk) • Gravimetric methods (AOAC 930.28- lactose in milk) • Mid-infrared spectrophotometric method (AOAC 972.16 and ISO 9622 |IDF 141) • Enzymatic methods (both AOAC and ISO/IDF) • GC methods • Capillary electrophoreses- Electrochemical detection (CE-ED) • HPLC-RI reference method for milk, dried milk and cream (ISO 22662 |IDF 198) • High performance anion-exchange chromatography (HPAEC) – pulsed amperometric detection (PAD) • HPLC-MS method

Low-Lactose SMPR Key Points

Applicability • Measure lactose found in milk , milk products, and  products containing dairy ingredients that are low  lactose or lactose free.  The analytical method must  account for potential interferences (See Table 1) in  these matrices.  This scope includes “lactose free”  infant formulas and adult nutritionals.