2018 August 26 SPSFAM Book

Regulations on Mycotoxins

China’s maximum levels for mycotoxins in foods GB 2761-2017 National Food Safety Standard Maximum Levels of Mycotoxins in Foods http://www.nhfpc.gov.cn/sps/s3594/201704/ee3109697fa24ee4bb7a1030c924f406.shtml EU mycotoxin regulations

Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs (consolidated version 2010-07-01) Regulation (EC) No 401/2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs (consolidated version 2010-03-13) https://ec.europa.eu/jrc/en/eurl/mycotoxins/legislation FDA mycotoxin regulatory levels Action Levels of Aflatoxins https://www.fda.gov/food/guidanceregulation/ucm077969.htm#afla Fumonisin Levels in Human Foods and Animal Feeds https://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/ChemicalContaminantsMetalsNaturalToxi nsPesticides/ucm109231.htm Advisory Levels for Deoxynivalenol https://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/ChemicalContaminantsMetalsNaturalToxi nsPesticides/ucm120184.htm

Trends in Mycotoxin Analysis

High

Multi-mycotoxins intrinsic properties

Single mycotoxin extrinsic properties

Future Automated and integrated sample preparation

Clean up (dilution)

GC-MS (/MS) Extraction (addition of I.S.)

Is LC-MS a viable approach for mycotoxin analysis?

TLC Soxhlet extraction (chloroform- methanol) SPE clean-up

ELISA (screening only) Extraction (MeOH/H 2 O: 7/3) Blend/shake/filter Adjust pH Color development MS confirmation

HPLC-UV/FLD Extraction SPE/ Immunoaffinity Cleanup Pre/Post column derivatization UV/FLD (MS confirmation)

General screening

Target analysis for confirmation and quantitation

Salting out

Clean up

(simplicity, speed)

Derivatization

Silica gel adsorbent (G60/chloroform /acetone- methanol) Visible/UV

Improvement in Sample preparation

Full scan, SIM, MS/MS

Low

Improvement in Instrumentation (sensitivity, specificity, data acquisition/processing, data mining )

High