AOAC ERP Gluten Assays - Dec 2018
Annex B
3
Materials and Methods
3.1
Test Kit Information
3.1.1 3.1.2
Kit Name
RIDASCREEN ® Total Gluten
Catalogue Number R7041
Ordering Information USA R-Biopharm Inc. 870 Vossbrink Drive
Washington, MO 63090 phone (269)-789-3033 fax (269)-789-3070 e-mail sales@r-biopharm.com Germany R-Biopharm AG An der neuen Bergstrasse 17 D-64297 Darmstadt Germany phone +49-(0)6151-8102-0 fax +49-(0)6151-8102-20 e-mail sales@r-biopharm.de
Further information is available at the web site address: www.r-biopharm.com
3.1.3 Reagents The test kit consists of antibody-coated microwell strips, standards, antibody conjugate, substrate/chromogen, stop solution, buffer, wash buffer (see 3.1.4-3.1.10); All reagents are stable as indicated on the label at 2-8°C (36-46°F). 3.1.4 Antibody-coated microwell strips. 3.1.5 Standards. – Six vials (1.3 mL each, ready to use) gluten proteins in aqueous solution. 3.1.6 Conjugate. – One vial (11 mL, ready to use), peroxidase conjugated antibody solution. 3.1.7 Red Chromogen Pro (Substrate/Chromogen). – One vial (13 mL, stained red). 3.1.8 Stop solution. – One vial (14 mL, contains 1 N sulfuric acid). 3.1.9 Buffer. – One bottle (120 mL, ready to use). 3.1.10 Wash buffer. – One bottle (100 mL, 10fold concentrate).
3.2
Additional Supplies and Reagents
3.2.1 3.2.2 3.2.3 3.2.4 3.2.5 3.2.6 3.2.7
Variable micropipettes (20 – 200 µL and 200 – 1000 µL)
Multistepper pipette and tips for 100 µl
8-channel pipette and tips for 100 µl and 250 µl Graduated pipettes (10 mL and 50 mL) Graduated cylinders (plastic or glass ware)
Distilled water
Ethanol; 99% reagent grade
3.2.8 Cocktail (patented); R7006/R7016 (R-Biopharm AG, Germany); ready to use 3.2.9 Skim milk powder (food quality; gluten-free) 3.2.10 Centrifugal glass vials with a screw top 3.2.11 Fluted paper filter
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RIDASCREEN ® Total Gluten In-house validation report 2018-10-21
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