AOAC ERP Gluten Assays - Dec 2018

Annex B

Table 1. Compounds tested for cross-reactivity and results of OD readings of these extracts compared to standard 1 (0 mg/kg gluten) and standard 2 (5 mg/kg gluten); compounds marked with an asterisk were extracted with addition of 1 g of gluten-free skim milk powder to mask polyphenols and other interfering substances.

OD Standard 1

OD Standard 2

OD Sample

Cereals Amaranth Arrowroot

0.143 0.187 0.143 0.187 0.106 0.161 0.161 0.187 0.187 0.228 0.160 0.143 0.106 0.143 0.187 0.161 0.161 0.161 0.161 0.161 0.161 0.161 0.161 0.161 0.161 0.111 0.161 0.228 0.228 0.111 0.143 0.143 0.161 0.161 0.161 0.228

0.414 0.444 0.414 0.444 0.429 0.426 0.426 0.444 0.444 0.445 0.443 0.414 0.429 0.414 0.444 0.426 0.426 0.426 0.426 0.426 0.426 0.426 0.426 0.426 0.426 0.390 0.426 0.445 0.445 0.390 0.414 0.414 0.426 0.426 0.426 0.445

0.112 0.116 0.225 0.114 0.255 0.159 0.177 0.141 0.243 0.265 0.238 0.130 0.214 0.234 0.242 0.186 0.152 0.151 0.153 0.151 0.178 0.173 0.168 0.173 0.192 0.113 0.201 0.238 0.219 0.212 0.200 0.269 0.164 0.176 0.218 0.255

Buckwheat, flour*

Chestnut, flour*

Corn starch

Millet, flour* Quinoa, flour

Rice flour, sweet Rice flour, white

Tapioca, flour

Teff, flour*

Beans/ Lentils/ Peas Black bean, flour

Chick peas Fava beans

Garbanzo beans

Guar gum

Green beans

Green pea, flour

Lentil, flour

Lima bean, flour

Lupine, flour

Pea flour, yellow

Peanut, raw

Peanut, roasted

Romano bean, flour

Sorghum, flour

Soya, flour

Soya milk

Soya protein

White bean, flour Seeds Carob, seedlings

Flax seed Pistachio

Poppy seed

Sesame, flour

Sunflower kernel

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RIDASCREEN ® Total Gluten In-house validation report 2018-10-21