AOAC ERP Gluten Assays - Dec 2018

Annex B

Table 9. Verification of LoQ in flakes, cereals, and flours by extracting 10 replicates of the blank samples and spiked in n=10 at a 2 mg/kg and 4 mg/kg gluten level; for each matrix and test kit lot the mean concentration, standard deviation and coefficient of variation is calculated; the recovery is calculated by using the mean concentration values (corrected for blank samples).

Flours

Lot 1

Lot 2

Flakes

Lot 1

Lot 2

Cereals

Lot 1

Lot 2

mg/kg

mg/kg

mg/kg

mg/kg

mg/kg

mg/kg

3.21 3.12 2.39 2.49 2.94 3.16 3.09 3.18 4.76 3.03 3.14 0.64 20.4

2.38 2.42 2.00 1.91 2.24 2.53 2.20 2.06 4.09 2.26 2.41 0.62 25.8

2.58 2.73 2.82 2.73 2.51 3.01 2.92 3.16 2.76 2.83 2.67 0.13

1.76 1.82 1.84 2.02 1.91 2.13 2.02 2.00 1.80 1.89 1.87 0.10

5.94 5.82 5.73 5.44 5.31 5.42 4.95 5.44 5.50 5.40 5.50 0.28 6.84 6.21 7.99 6.04 6.50 6.48 6.95 7.42 7.06 6.95 6.84 0.61 8.59 8.73 8.41 9.69 7.97 7.30 8.25 7.97 8.85 1.23 13.9 5.1 9.0 67

3.70 4.03 3.61 3.28 3.88 3.39 3.37 3.66 3.24 3.80 3.60 0.27 5.26 4.85 7.02 4.58 4.74 4.70 5.07 5.87 5.38 5.11 5.26 0.77 14.7 8.72 6.45 6.90 6.64 7.59 7.02 6.47 6.53 6.45 6.97 6.97 0.75 10.8 7.4 83

blank

blank

blank

mean

SD

4.7

5.3

CV [%]

Spike 2 mg/kg

Spike 2 mg/kg

Spike 2 mg/kg

Not tested

Not tested

mean

SD

CV [%]

mean rec. [%]

4.89 6.55 6.00 5.32 5.64 5.17 5.53 5.51 6.76 6.63 5.68 0.64 11.2

4.29 6.27 6.40 5.47 5.93 5.32 5.69 5.56 7.08 6.60 6.17 0.47

5.81 6.49 5.90 6.84 5.79 5.68 5.87 5.68 5.72 5.72 5.67 0.85 15.0

5.82 6.44 5.78 6.74 5.28 5.36 5.25 5.39 5.52 5.49 6.01 0.58 9.6 104

11.36

Spike 4 mg/kg

Spike 4 mg/kg

Spike 4 mg/kg

10.27

mean

SD

7.7

CV [%]

64

87

82

84

84

mean rec. [%]

Page 21 of 33

RIDASCREEN ® Total Gluten In-house validation report 2018-10-21