AOAC ERP Gluten Assays - Dec 2018

Annex B

Table 12. Analysis of the oat Reference Materials mentioned in AOAC SMPR ® 2017.021 to characterize the RIDASCREEN ® Total Gluten for trueness at a gluten level of 20 mg/kg; 10 replicates in three different lots.

Lot 1

Lot 2

Lot 3

mg/kg

mg/kg

mg/kg

17.9 21.1 17.3 20.3 18.5 18.6 20.4 19.1 20.9 18.6 19.3 27.5 23.8 29.8 30.8 32.3 24.6 27.3 26.1 25.8 24.8 27.3 137 2.84 10.4 15.9 12.2 14.8 19.2 15.0 18.5 21.7 16.2 16.0 26.5 97 1.32 6.8

17.0 19.6 16.9 20.6 18.7 19.5 20.1 18.4 18.6 17.8 18.7 32.2 28.7 34.6 34.3 34.4 26.5 28.2 28.5 30.6 29.2 30.7 154 20.1 16.2 17.7 22.4 18.1 32.0 18.7 19.6 19.0 28.7 21.2 106 5.11 24.1 94 1.25 6.7 2.97 9.7

19.9 22.1 17.8 21.2 17.8 18.4 21.0 20.1 21.3 19.7 19.9 100 28.0 24.5 31.5 30.7 32.3 24.1 26.2 27.4 27.0 25.6 27.7 139 2.89 10.4 18.2 14.9 17.3 20.4 18.7 21.9 20.2 18.9 21.9 27.0 19.9 100 3.27 16.4 1.52 7.6

Wheat in oat 20 mg/kg

Mean

Mean rec. [%]

SD

CV [%]

Rye in oat 20 mg/kg

Mean

Mean rec. [%]

SD

CV [%]

Barley in oat 20 mg/kg

Mean

17.6

88

Mean rec. [%]

SD

4.09 23.2

CV [%]

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RIDASCREEN ® Total Gluten In-house validation report 2018-10-21