AOAC ERP Gluten Assays - Dec 2018

Annex B

Table 13. Recovery experiment for oat-based cookies incurred with wheat or rye or barley at gluten levels of 11.5 mg/kg or 46 mg/kg; six extractions per lot and sample

Lot 1

Lot 2

Lot 3

mg/kg gluten

Recovery

mg/kg gluten

Recovery

mg/kg gluten

Recovery

1.11 1.11 7.13 8.39 6.91 6.57 13.4 6.93 12.3 14.9 12.5 10.3 10.8 15.2 14.9 12.9 15.3 12.7 15.7 23.5 27.2 25.7 25.3 27.0 31.7 45.1 37.0 41.9 39.0 42.0 40.5 39.0 43.8 43.0 41.3 42.8 35.7

0.69 1.06 6.38 7.05 5.82 5.76 12.0 5.72 11.8 13.6 12.7 10.1 10.6 15.7 14.7 13.6 15.7 14.2 17.3 23.3 24.6 24.8 24.1 25.4 27.3 43.5 34.8 40.1 36.4 39.0 38.1 39.2 45.1 43.8 41.8 43.6 38.5

0.64 0.98 6.61 7.44 5.76 5.54 11.1 6.02 10.5 12.6 10.8 9.32 9.16 12.3 11.9 11.1 13.4 11.4 13.5 21.1 23.6 23.0 22.1 24.5 27.1 39.3 30.8 35.4 32.6 34.9 33.6 32.7 37.5 36.0 34.7 35.5 31.6

Blank

62% 73% 60% 57%

55% 61% 51% 50%

57% 65% 50% 48% 96% 52% 91% 94% 81% 80%

Wheat gluten 11.5 mg/kg

117%

104%

60%

50%

107% 130% 109%

102% 119% 110%

110%

Rye gluten 11.5 mg/kg

90% 94%

88% 92%

44.5*

na

44.8*

na

37.2*

na

132% 129% 112% 133% 111% 137%

137% 128% 118% 137% 124% 151%

107% 103%

97%

Barley gluten 11.5 mg/kg

116%

99%

118%

51% 59% 56% 55% 59% 69% 98% 81% 91% 85% 91% 88% 85% 95% 93% 90% 93% 78%

51% 54% 54% 52% 55% 59% 95% 76% 87% 79% 85% 83% 85% 98% 95% 91% 95% 84%

46% 51% 50% 48% 53% 59% 85% 67% 77% 71% 76% 73% 71% 82% 78% 76% 77% 69%

Wheat gluten 46 mg/kg

Rye gluten 46 mg/kg

Barley gluten 46 mg/kg

Page 26 of 33

RIDASCREEN ® Total Gluten In-house validation report 2018-10-21