AOAC ERP Gluten Assays - Dec 2018

Annex A

Table A-1. Raw concentration data in mg/kg gluten for blind duplicates from 19 participants using the 4-parameter logistic regression for calculation; data presented were calculated from the mean of two replicates on the plate per sample; part 1 for samples 1 - 10; outliers are marked as c for Cochran, g for Grubbs, dg for double Grubbs. means of duplicate wells and the two values per sample are those of the blind duplicates

Lab

1

2

3

4

5

6

7

8

9

10

A B C

12.3 8.6 10.8 12.6 15.9 11.8 8.4 10.4 11.77c 0.1c 12.3 10.7

0.4 0.1 0.4

2.2 0.1 0.4

64.3 62.5 33.4 40.7 2.7 50.5 59.9 30.6 14.7 1.6 63.0 70.4 29.8 28.1 4.6

2.6 5.4 8.8 30.0 31.2 47.6 55.2 1.6 6.9 14.1 22.0 28.6 42.6 32.3 4.3 10.5 9.1 24.1 22.8 41.0 37.4

15.4 8.6 12.6 16.7 7.7

8.1

D 10.6 11.9 11.5 12.6 10.2 12.4 0.6c 5.8c 119.5c 53.6c 43.4 28.3 35.4c 15.1c 13.3 8.3 25.3 38.6 57.7 28.1 E 10.5 9.1 14.2 16.5 11.8 11.2 0.4 0.2 57.1 62.2 30.4 31.6 5.7 4.7 6.4 8.7 29.3 26.0 49.1 49.1 F 12.1 10.3 11.6 12.5 10.0 9.5 3.3 0.1 57.0 53.1 32.7 29.2 4.4 5.8 8.1 6.2 31.0 27.8 40.2 47.0 G 10.1 9.4 12.9 17.4 12.9 11.0 0.2 0.5 59.2 64.9 36.0 35.1 7.8 7.5 5.0 5.6 31.2 32.9 45.0 49.0 H 12.8 9.2 13.0 13.7 10.8 12.2 0.5 2.8 68.1 75.1 31.9 37.6 8.0 8.8 68.2 10.1 42.2 45.5 67.3 49.0 I 12.5 8.4 14.3 14.9 11.2 13.7 0.3 1.1 62.1 75.8 36.5 37.2 7.3 9.1 7.8 8.7 41.8 45.3 62.9 61.6 J 10.2 9.4 18.4 13.0 9.9 13.3 0.3 0.9 63.1 63.1 29.3 33.8 1.2 3.0 7.7 6.3 39.1 37.0 49.9 55.0 K 11.5 6.0 13.0 10.6 9.9 9.9 0.9c 14.1c 50.4 58.3 53.3 47.8 1.0 3.5 6.8 6.2 24.5 51.3 43.1 46.3 L 10.8 15.5 13.1 14.5 22.0g 20.3g 0.8 1.2 77.1 62.9 32.9 25.8 7.1c 0.5c 12.5 8.1 44.4 35.3 45.5 50.8 M 10.2 9.2 11.8 9.8 8.7 8.6 1.7 0.1 53.2 50.7 24.5 25.0 2.1 1.0 7.6 5.9 25.5 27.6 40.5 44.3 N 10.9 11.2 16.4 11.6 10.1 10.4 1.3 0.8 68.5 65.1 28.3 36.0 5.4 6.1 9.3 9.8 33.3 30.3 48.2 50.5 O 12.2 12.5 13.5 14.9 12.0 9.5 1.7 0.6 61.8 63.9 34.4 36.9 2.9 3.6 8.0 7.5 40.0 28.6 47.7 41.4 P 12.8 10.5 14.6 15.8 16.5g 11.7g 3.7dg 6.7dg 65.6 49.6 27.1 33.1 6.4c 11.2c 15.9 8.5 29.8 23.0 46.9 62.4 Q 12.8 11.1 15.6 15.5 10.2 10.8 2.2 0.7 57.2 57.7 38.9 37.1 2.6 1.8 9.7 8.7 33.3 33.6 49.7 41.0 R 10.6 10.4 10.5 14.0 8.4 11.3 1.7 0.6 58.9 59.2 33.6 28.9 1.0 1.1 7.0 6.5 32.9 27.0 51.0 45.4 U 8.8 14.8 14.5 14.9 13.8 15.6 3.3dg 4.6dg 74.4 71.3 39.1 38.2 3.2 2.3 7.6 9.9 38.3 35.8 48.7 51.2

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