AOAC ERP Gluten Assays - Dec 2018
14. Add 100 µL of stop reagent to each well. Mix gently by shaking the plate manually and measure the absorbance at 450 nm against an air blank. Read within 10 minutes after addition of the stop solution. Write down the absorbances in the data return sheet 15. Check the shape of the standard curve (not the precise OD‐values) if it is similar to the Quality Assurance Certificate enclosed in the test kit. 16. Please document all results in the data return sheet. Document any changes to this protocol in the data return sheet as well. 17. Send the data return sheet to the study director (k.scherf.leibniz‐lsb@tum.de). 18. Continue with the next step after the Study Director has approved the data of the Pre‐ Test.
6
Made with FlippingBook - Online catalogs